Biology Lab Report - 902 Words

Abstract: Background: High blood glucose fluctuation (BGF) contributes in chronic complications by promoting the dysfunction of vascular endothelium in diabetes. MicroRNA (miR)-34 family is known for its roles in the pathophysiology of diverse diseases including type 2 diabetes. However, the action of BGF on the expression of these miRs in diabetes is unknown. Herein, we aimed to investigate the effects of BGF on the pathogenesis of diabetes and the possible involvement of miR-34 family in vivo. Methods: A total of 60 Sprague-Dawley (SD) rats were randomly assigned to four groups with 15 rats per group, namely normal control (NC) group, model control (MC) group, persistent high blood glucose (PHG) group and fluctuant high blood glucose†¦show more content†¦Keywords: Diabetes, persistent high blood glucose, blood glucose fluctuation, miR-34b/c. Background Diabetes is a chronic metabolic disorder, which seriously affects people’s health 1. The prevalence of diabetes is approximately 9.7%, accounting for 92.4 million adults in China 2. Type 2 diabetes has become the most frequent type of diabetes and is commonly related with the prevalence of obesity, aging and hyperinsulinemia 3. Moreover, Type 2 diabetes is a major risk factor for cardiovascular diseases and increases the mortality of these patients 4, 5. The risk of cardiovascular diseases increases for subjects with postprandial hyperglycemia 6, 7. However, it is difficult to evaluate the relationship between postprandial high blood glucose and cardiovascular disease because the patients with postprandial hyperglycemia are often accompanied with hyperinsulinemia, insulin resistance and postprandial dyslipidemia 8. Several other mechanisms causing cardiovascular complications in diabetic patients including glucose toxicity, impaired insulin growth factor (IGF) signaling and insulin receptor, vascular inflammation and cerebrovascular injury have also been proposed 9, 10. However, the underlying molecular mechanisms are still far from being completely clarified. Several large clinical studies, such as the United Kingdom Prospective Diabetes Study (UKPDS) and the Diabetes Control and Complications Trial (DCCT), have suggested a positive correlationShow MoreRelatedBiology Lab : Lab Report Essay777 Words   |  4 PagesLab 9: Pea Genetics Lab Report Zach Freeland : Section 03 : BIOS 210 Fundamentals of Cellular Biology Lab Title: †¢ Gathering Data on the Different Traits of the Garden Pea (Pisum Sativum), Organizing the Dominant/Recessive Phenotypes of 60 F2 Offspring and Determining Whether the Null Hypothesis is Rejected or Accepted Using the Chi-Square Test. Introduction: †¢ This lab had 2 exercises. Exercise 9.1 involved observing pictures of 60 F2 offspring and recording the phenotypes for 6 different traitsRead MoreBiology Lab Report840 Words   |  4 PagesScreening and Cloning Of Bacterial ÃŽ ²-Glucosidase Gene That Can Degrade Salicin from NIF and Virulent Bacteria Hanan H. Ahmed Microbial Biotechnology Department, Genetic Engineering Biotechnology Institute, Minufiya University Sadat City, Egypt Abstract Two ÃŽ ²- glucosidase genes in Rhizobium leguminosarum bv. Trifloii able to utilize Salicin. SamI fragments (2 and 3 kbp) from Rhizobium leguminosarum bv. Trifloii were expressed in E. coli HC1061. Transformed clones with ÃŽ ²- glucosidase activityRead MoreBiology Lab Report797 Words   |  4 Pageswas not significant enough to make comparisons and was subject to error. Valid relationships between the tested variables could not be supported and the hypothesis presented in this study was not proven. 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Hence secular variation of elemental and concentrations and isotopic compositions of seawater can reflect major changes in the lithosphere and the atmosphere over geologic time. Consequently, elemental and isotopic composition of marine chemical sediments (e.g., carbonatesRead MoreBiology Beetroot Lab Report2895 Words   |  12 Pagesusing this knowledge gained. A risk assessment has been conducted in the process of planning this investigation and the possible risks may be glass breakage or burnt by hot water. These dangers can be prevented with more careful actions around the lab. It is also essential that safety glasses are worn during the entire course of the experiment. Method: 1. 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